ARG63290

anti-BERP / RNF22 antibody

anti-BERP / RNF22 antibody for Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody

Overview

Product Description Goat Polyclonal antibody recognizes BERP / RNF22
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Cow, Dog
Tested Application WB
Specificity Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name BERP / RNF22
Antigen Species Human
Immunogen AKREDSPGPEVQP-C
Conjugation Un-conjugated
Alternate Names BERP; RING finger protein 97; RNF97; HAC1; Brain-expressed RING finger protein; Tripartite motif-containing protein 3; RING finger protein 22; RNF22

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10612 Human TRIM3

GeneID: 55992 Mouse TRIM3

GeneID: 83616 Rat TRIM3

Background The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody
Calculated MW 81 kDa