ARG41307

anti-BHLHB2 antibody

anti-BHLHB2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes BHLHB2
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Gpig, Hrs, Rb, Sheep
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name BHLHB2
Antigen Species Human
Immunogen Synthetic peptide around the middle region of Human BHLHB2. (within the following region: SEKGDLRSEQPCFKSDHGRRFTMGERIGAIKQESEEPPTKKNRMQLSDDE)
Conjugation Un-conjugated
Alternate Names Enhancer-of-split and hairy-related protein 2; bHLHb2; Differentially expressed in chondrocytes protein 1; bHLHe40; HLHB2; Class B basic helix-loop-helix protein 2; BHLHB2; Class E basic helix-loop-helix protein 40; Stra14; STRA13; Stimulated by retinoic acid gene 13 protein; DEC1; SHARP-2

Application Instructions

Predict Reactivity Note Predicted Homology Based On Immunogen Sequence: Cow: 92%; Dog: 93%; Guinea pig: 100%; Horse: 100%; Mouse: 100%; Rabbit: 86%; Rat: 100%; Sheep: 92%
Application Suggestion
Tested Application Dilution
IHC-P4 - 8 µg/ml
WB1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human fetal lung

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS, 0.09% (w/v) Sodium azide and 2% Sucrose.
Preservative 0.09% (w/v) Sodium azide
Stabilizer 2% Sucrose
Concentration Batch dependent: 0.5 - 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8553 Human BHLHE40

Swiss-port # O14503 Human Class E basic helix-loop-helix protein 40

Gene Symbol BHLHE40
Gene Full Name basic helix-loop-helix family, member e40
Background This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. [provided by RefSeq, Feb 2014]
Function Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May be involved in the regulation of chondrocyte differentiation via the cAMP pathway. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Note=Predominantly localized in the nucleus (PubMed:11278694). [UniProt]
Calculated MW 46 kDa
PTM Ubiquitinated; which may lead to proteasomal degradation.

Sumoylation inhibits its ubiquitination and promotes its negative regulation of the CLOCK-ARNTL/BMAL1 heterodimer transcriptional activator activity. [UniProt]