ARG63573
anti-BIM antibody
anti-BIM antibody for Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes BIM |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise at least reported isoforms Bim-alpha1 (BimABCD), Bim-alpha2 (BimACD) and Bim-alpha3 (BimAD). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BIM |
| Antigen Species | Human |
| Immunogen | C-FNAYYARRLEK |
| Conjugation | Un-conjugated |
| Alternate Names | Bcl2-L-11; BIM; Bcl2-interacting mediator of cell death; Bcl-2-like protein 11; BAM; BOD |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 22 kDa |
| PTM | Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation (PubMed:15486195, PubMed:21478148). Deubiquitination catalyzed by USP27X stabilizes the protein (By similarity). Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein. |
