ARG41883

anti-BIRC3 / cIAP2 antibody

anti-BIRC3 / cIAP2 antibody for Western blot,ICC/IF and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes BIRC3 / cIAP2
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name BIRC3 / cIAP2
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-180 of Human BIRC3 / cIAP2. (NP_001156.1)
Conjugation Un-conjugated
Alternate Names MIHC; EC 6.3.2.-; hIAP1; API2; HIAP1; Baculoviral IAP repeat-containing protein 3; Inhibitor of apoptosis protein 1; hIAP-1; IAP homolog C; CIAP2; RNF49; C-IAP2; IAP-1; RING finger protein 49; HAIP1; c-IAP2; TNFR2-TRAF-signaling complex protein 1; Apoptosis inhibitor 2; MALT2; AIP1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:200 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse lung
Observed Size ~ 80 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11796 Mouse BIRC3

GeneID: 330 Human BIRC3

Swiss-port # O08863 Mouse Baculoviral IAP repeat-containing protein 3

Swiss-port # Q13489 Human Baculoviral IAP repeat-containing protein 3

Gene Symbol BIRC3
Gene Full Name baculoviral IAP repeat containing 3
Background This gene encodes a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified. [provided by RefSeq, Aug 2011]
Function Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, IKBKE, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. [UniProt]
Cellular Localization Cytoplasm. Nucleus. [UniProt]
Calculated MW 68 kDa
PTM Auto-ubiquitinated and degraded by the proteasome in apoptotic cells. [UniProt]