ARG54438
anti-BIRC7 / LIVIN antibody
anti-BIRC7 / LIVIN antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes BIRC7 / LIVIN |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | This antibody recognizes human Livin (33kDa). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BIRC7 / LIVIN |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to aa 264-280 of the short form and aa 281-298 of the long form of human Livin (accession no. NP_071444). |
| Conjugation | Un-conjugated |
| Alternate Names | p30-Livin; ML-IAP; Baculoviral IAP repeat-containing protein 7; EC 6.3.2.-; RNF50; Melanoma inhibitor of apoptosis protein; Livin; tLivin; Kidney inhibitor of apoptosis protein; Truncated livin; RING finger protein 50; KIAP; MLIAP; LIVIN |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Raji and Human small intestine |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q96CA5 Human Baculoviral IAP repeat-containing protein 7 |
|---|---|
| Gene Symbol | BIRC7 |
| Gene Full Name | baculoviral IAP repeat containing 7 |
| Background | Apoptosis is prevented by the inhibitor of apoptosis (IAP) proteins. A novel member in the IAP protein family has been identified and designated Livin and KIAP for kidney IAP. Livin/KIAP contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin-a and Livin-b. Transfection of Livin in cells results in protection from apoptosis induced by FADD, BAX, RIP, RIP3 and DR6. Livin has direct interaction with several caspases including caspase-3, -7, and -9. Livin inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. The two isoforms of Livin appear to have different functions and tissue distributions. |
| Function | Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody |
| Calculated MW | 33 kDa |
| PTM | Autoubiquitinated and undergoes proteasome-mediated degradation. The truncated protein (tLivin) not only loses its anti-apoptotic effect but also acquires a pro-apoptotic effect. |
