ARG66736

anti-BLM / Blooms Syndrome Protein antibody

anti-BLM / Blooms Syndrome Protein antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes BLM / Blooms Syndrome Protein
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name BLM / Blooms Syndrome Protein
Antigen Species Human
Immunogen Synthetic peptide between aa. 65-114 of Human BLM / Blooms Syndrome Protein.
Conjugation Un-conjugated
Alternate Names Bloom syndrome protein; RECQL3; RECQL2; RECQ2; BS; RecQ protein-like 3; EC 3.6.4.12; DNA helicase, RecQ-like type 2; RecQ2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:1000
IHC-P1:100 - 1:300
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 641 Human BLM

Swiss-port # P54132 Human Bloom syndrome protein

Gene Symbol BLM
Gene Full Name Bloom syndrome, RecQ helicase-like
Background The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008]
Function ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed:9388193, PubMed:24816114, PubMed:25901030). Participates in DNA replication and repair (PubMed:12019152, PubMed:21325134, PubMed:23509288). Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:21325134). Negatively regulates sister chromatid exchange (SCE) (PubMed:25901030). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution (PubMed:25901030). Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction (PubMed:20639533, PubMed:24257077, PubMed:25901030). [UniProt]
Cellular Localization Nucleus. Note=Together with SPIDR, is redistributed in discrete nuclear DNA damage-induced foci following hydroxyurea (HU) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a RMI complex- and SPIDR-dependent manner. [UniProt]
Calculated MW 159 kDa
PTM Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1. [UniProt]