ARG58061

anti-BOB1 / OCAB / OBF1 antibody

anti-BOB1 / OCAB / OBF1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes BOB1 / OCAB / OBF1
Tested Reactivity Hu
Tested Application IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name BOB1 / OCAB / OBF1
Antigen Species Human
Immunogen Synthetic peptide derived from Human BOB1.
Conjugation Un-conjugated
Alternate Names POU domain class 2-associating factor 1; OCA-B; BOB-1; OCAB; OBF-1; OBF1; OCT-binding factor 1; B-cell-specific coactivator OBF-1; BOB1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:500
IP1:30
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Ramos
Observed Size ~ 35 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5450 Human POU2AF1

Swiss-port # Q16633 Human POU domain class 2-associating factor 1

Gene Symbol POU2AF1
Gene Full Name POU class 2 associating factor 1
Function Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. [UniProt]
Calculated MW 27 kDa
PTM Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. [UniProt]