ARG57102

anti-Bak antibody [38E2]

anti-Bak antibody [38E2] for ICC/IF,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Cell Death antibody; Pro-apoptotic Bcl2 protein antibody

Overview

Product Description Mouse Monoclonal antibody [38E2] recognizes Bak
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone 38E2
Isotype IgG2a, kappa
Target Name Bak
Antigen Species Human
Immunogen Recombinant fragment around aa. 29-187 of Human Bak
Conjugation Un-conjugated
Alternate Names Apoptosis regulator BAK; BCL2L7; Bcl-2-like protein 7; CDN1; Bcl2-L-7; Bcl-2 homologous antagonist/killer; BAK-LIKE; BAK

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WB1:250 - 1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12018 Mouse BAK1

GeneID: 578 Human BAK1

Swiss-port # O08734 Mouse Bcl-2 homologous antagonist/killer

Swiss-port # Q16611 Human Bcl-2 homologous antagonist/killer

Gene Symbol BAK1
Gene Full Name BCL2-antagonist/killer 1
Background The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]
Function In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Pro-apoptotic Bcl2 protein antibody
Calculated MW 23 kDa