ARG62367
anti-Bak antibody
anti-Bak antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Hamster,Human,Mouse
Cell Biology and Cellular Response antibody; Cell Death antibody; Pro-apoptotic Bcl2 protein antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Bak |
|---|---|
| Tested Reactivity | Hu, Ms, Hm |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Bak |
| Antigen Species | Human |
| Immunogen | A synthetic peptide from the N terminus of human Bak protein. |
| Epitope | N-terminal |
| Conjugation | Un-conjugated |
| Alternate Names | Apoptosis regulator BAK; BCL2L7; Bcl-2-like protein 7; CDN1; Bcl2-L-7; Bcl-2 homologous antagonist/killer; BAK-LIKE; BAK |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | A431 cells. Tonsil. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified Antibody |
| Buffer | 1X PBS and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O08734 Mouse Bcl-2 homologous antagonist/killer Swiss-port # Q16611 Human Bcl-2 homologous antagonist/killer |
|---|---|
| Gene Symbol | BAK1 |
| Gene Full Name | BCL2-antagonist/killer 1 |
| Background | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008] |
| Function | In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30275 Pro-apoptotic Bcl2 protein Antibody Panel (BAX, BAK, Bid) Related products: Bak antibodies; Bak Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Pro-apoptotic Bcl2 protein antibody |
| Calculated MW | 23 kDa |
Customer's Feedback
Good
Review for anti-Bak antibody
Application：WB
Sample：Mouse lung
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-Bak antibody
Application：WB
Sample：Jurkat
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
