ARG63187

anti-Bak antibody

anti-Bak antibody for Western blot and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Pro-apoptotic Bcl2 protein antibody

Overview

Product Description Goat Polyclonal antibody recognizes Bak
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Pig
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Bak
Antigen Species Human
Immunogen ASGQGPGPPRQE-C
Conjugation Un-conjugated
Alternate Names Apoptosis regulator BAK; BCL2L7; Bcl-2-like protein 7; CDN1; Bcl2-L-7; Bcl-2 homologous antagonist/killer; BAK-LIKE; BAK

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.5 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 578 Human BAK1

Swiss-port # Q16611 Human Bcl-2 homologous antagonist/killer

Background The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Pro-apoptotic Bcl2 protein antibody
Calculated MW 23 kDa