ARG65612

anti-Bax antibody

anti-Bax antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondrial fission antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes Bax

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Bax
Antigen Species Human
Immunogen Fusion protein of human BAX
Conjugation Un-conjugated
Alternate Names Bcl-2-like protein 4; Bcl2-L-4; BCL2L4; Apoptosis regulator BAX

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:20 - 1:100
IHC-P1:25 - 1:100
WB1:500 - 1:3000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol
Preservative 0.05% Sodium azide
Stabilizer 40% Glycerol
Concentration 1.1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12028 Mouse BAX

GeneID: 581 Human BAX

Swiss-port # Q07812 Human Apoptosis regulator BAX

Swiss-port # Q07813 Mouse Apoptosis regulator BAX

Gene Symbol BAX
Gene Full Name BCL2-associated X protein
Background Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Dec 2019]
Function Bax plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30263 Mitochondrial fission Antibody Duo (Drp1, BAX)
ARG30268 Apoptosis Marker Antibody Duo (Bcl2, Bax)
Related products:
Bax antibodies; Bax Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Understanding Your Cells: Choose the right markers
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondrial fission antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody
Calculated MW 21 kDa

Customer's Feedback

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Review for anti-Bax antibody

Application:WB

Sample:U87

Sample Loading Amount:20 µl

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-Bax antibody

Application:IF/ICC

Sample:HeLa

Fixation Buffer:Methanol / Acetone 1:1 ratio

Fixation Time:20 min

Fixation Temperature:-20 ºC

Antigen Retrieval Buffer:N/A

Antigen Retrieval Time:N/A

Antigen Retrieval Temperature:N/A ºC

Primary Antibody Dilution Factor:1:20

Primary Antibody Incubation Time:1 hour

Primary Antibody Incubation Temperature:RT ºC

Specific References

Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions

WB / Human

Peng-Hsiang Fang et al.
Molecular Medicine,  (2022)

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Preclinical Therapeutic Assessment of a New Chemotherapeutics [Dichloro(4,4'-Bis(2,2,3,3-Tetrafluoropropoxy) Methyl)-2,2'-Bipryridine) Platinum] in an Orthotopic Patient-Derived Xenograft Model of Triple-Negative Breast Cancers

WB / Human

Tzu-Chun Kan et al.
Pharmaceutics.,  (2022)

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Kansuinine A Ameliorates Atherosclerosis and Human Aortic Endothelial Cell Apoptosis by Inhibiting Reactive Oxygen Species Production and Suppressing IKKβ/IκBα/NF-κB Signaling

WB / Human

Chen-Sheng Chen et al.
Int J Mol Sci.,  (2021)

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Multi-omics analysis of pathological changes in the amygdala of rats subjected to chronic restraint stress.

WB / Rat

Zhonghua Li et al.
Behav Brain Res.,  (2020)

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A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis

WB / Mouse

Xuan Wang et al.
Oncol Lett.,  (2018)

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