ARG55935

Name: anti-Bcl 2 antibody [124]
Brand: arigo Biolaboratories
SKU: ARG55935
Price: 329 USD
Availability: InStock
Rating: 4.0 (1 reviews)

anti-Bcl 2 antibody [124]

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Price (USD)

50 μg

329.00

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Datasheet
Review (1)

Overview

Product Description	Mouse Monoclonal antibody [124] recognizes Bcl 2
Tested Reactivity	Hu
Species Does Not React With	Ms, Rat
Tested Application	FACS, ICC/IF, IHC-P, WB
Host	Mouse
Clonality	Monoclonal
Clone	124
Isotype	IgG1, kappa
Target Name	Bcl 2
Antigen Species	Human
Immunogen	Synthetic peptide around aa. 41-54 of Human Bcl-2 protein (GAAPAPGIFSSQPG-C).
Conjugation	Un-conjugated
Alternate Names	Apoptosis regulator Bcl-2; Bcl-2; PPP1R50

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	1 - 2 µg/10^6 cells
ICC/IF	1 - 5 µg/ml
IHC-P	1 - 5 µg/ml
WB	0.5 - 1 µg/ml

Application Note

IHC-P: Antigen Retrieval: Boil tissue section in 1 mM EDTA buffer (pH 7.5-8.5) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Purification with Protein G.
Buffer	PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative	0.05% Sodium azide
Stabilizer	0.1 mg/ml BSA
Concentration	0.2 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 596 Human BCL2 Swiss-port # P10415 Human Apoptosis regulator Bcl-2
Gene Symbol	BCL2
Gene Full Name	B-cell CLL/lymphoma 2
Background	This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]
Function	Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). [UniProt]
Cellular Localization	Cytoplasmic, membrane
Highlight	Related products: Anti-Mouse IgG secondary antibodies; Related news: Lymphoma
Research Area	Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
Calculated MW	26 kDa
PTM	Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.