ARG65415
anti-Bcl 2 antibody [Bcl2/100/D5]
anti-Bcl 2 antibody [Bcl2/100/D5] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
1
Overview
| Product Description | Mouse Monoclonal antibody [Bcl-2/100/D5] recognizes Bcl 2 |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Ms |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | The clone Bcl2/100 recognizes Bcl2, a 26 kDa protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | Bcl2/100/D5 |
| Isotype | IgG1 |
| Target Name | Bcl 2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2 |
| Conjugation | Un-conjugated |
| Alternate Names | Apoptosis regulator Bcl-2; Bcl-2; PPP1R50 |
Application Instructions
| Application Suggestion |
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BCL2 |
| Gene Full Name | B-cell CLL/lymphoma 2 |
| Background | Bcl2 (B cell CLL lymphoma 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms. |
| Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). [UniProt] |
| Highlight | Related products: Anti-Mouse IgG secondary antibodies; Related news: Lymphoma |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody |
| Calculated MW | 26 kDa |
| PTM | Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. |
Customer's Feedback
Excellent
Review for anti-Bcl 2 antibody [Bcl2/100/D5]
Application：WB
Sample：Jurkat cell lysate
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
