ARG54027

anti-BiP / GRP78 antibody

anti-BiP / GRP78 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody
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Overview

Product Description

Mouse Monoclonal antibody recognizes BiP / GRP78

Tested Reactivity Hu, Rat
Tested Application ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Isotype IgG
Target Name BiP / GRP78
Antigen Species Human
Immunogen Purified recombinant human BiP/GRP78 protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names Heat shock 70 kDa protein 5; BiP; GRP-78; Endoplasmic reticulum lumenal Ca; MIF2; 78 kDa glucose-regulated protein; GRP78; Immunoglobulin heavy chain-binding protein; HEL-S-89n; BIP; 2+

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50
IHC-P1:100
WB1:1000
Application Note IHC-P: Antigen Retrieval: Pressure cooking in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 0.8 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25617 Rat HSPA5

GeneID: 3309 Human HSPA5

Swiss-port # P06761 Rat 78 kDa glucose-regulated protein

Swiss-port # P11021 Human 78 kDa glucose-regulated protein

Gene Symbol HSPA5
Gene Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Background Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER
Function Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. [UniProt]
Cellular Localization Endoplasmic reticulum lumen. Melanosome
Research Area Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody
Calculated MW 72 kDa

Customer's Feedback

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Review for anti-BiP / GRP78 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Endoplasmic reticulum stress promotes sorafenib resistance via miR-188-5p/hnRNPA2B1-mediated upregulation of PKM2 in hepatocellular carcinoma

WB / Human

Bei Zhou et al.
Mol Ther Nucleic Acids .,  (2021)

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Study of isolation and characterization of extracellular vesicles in senescent tumor cells induced by chemotherapeutics.

WB / Human

Qi Zhou et al.
Chinese Journal of Immunology.,  (2020)

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Melatonin Increases the Sensitivity of Hepatocellular Carcinoma to Sorafenib through the PERK-ATF4-Beclin1 Pathway

WB, ICC/IF / Human

Zhou Bei et al.
Int J Biol Sci.,  (2019)

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Effects of calpain inhibitor on the apoptosis of hepatic stellate cells induced by calcium ionophore A23187.

WB / Human

Li Yanan et al.
J Cell Biochem.,  (2018)

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Effects of calcium Ionophore A23187 on the apoptosis of hepatic stellate cells stimulated by transforming growth factor-β1.

WB / Rat

Li Yanan et al.
Cell Mol Biol Lett.,  (2018)

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