ARG55138

anti-BiP / GRP78 antibody

anti-BiP / GRP78 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes BiP / GRP78

Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name BiP / GRP78
Antigen Species Human
Immunogen Recombinant protein encoding full length Human BiP.
Conjugation Un-conjugated
Alternate Names Heat shock 70 kDa protein 5; BiP; GRP-78; Endoplasmic reticulum lumenal Ca; MIF2; 78 kDa glucose-regulated protein; GRP78; Immunoglobulin heavy chain-binding protein; HEL-S-89n; BIP; 2+

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14828 Mouse HSPA5

GeneID: 3309 Human HSPA5

Swiss-port # P11021 Human 78 kDa glucose-regulated protein

Swiss-port # P20029 Mouse 78 kDa glucose-regulated protein

Gene Symbol HSPA5
Gene Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Background The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
Function Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. [UniProt]
Cellular Localization Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Research Area Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody
Calculated MW 72 kDa