ARG57717

anti-BiP / GRP78 antibody

anti-BiP / GRP78 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody

Overview

Product Description Mouse Monoclonal antibody recognizes BiP / GRP78
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IP, WB
Specificity This antibody detects endogenous levels of BiP / GRP78 and does not cross-react with related proteins.
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Target Name BiP / GRP78
Antigen Species Human
Immunogen Purified recombinant Human BiP / GRP78 protein expressed in E. coli.
Conjugation Un-conjugated
Alternate Names Heat shock 70 kDa protein 5; BiP; GRP-78; Endoplasmic reticulum lumenal Ca; MIF2; 78 kDa glucose-regulated protein; GRP78; Immunoglobulin heavy chain-binding protein; HEL-S-89n; BIP; 2+

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size ~ 72 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.03% Proclin300 and 50% Glycerol.
Preservative 0.03% Proclin300
Stabilizer 50% Glycerol
Concentration 4.4 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14828 Mouse HSPA5

GeneID: 25617 Rat HSPA5

GeneID: 3309 Human HSPA5

Gene Symbol HSPA5
Gene Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Background The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
Function Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. [UniProt]
Research Area Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody
Calculated MW 72 kDa