ARG65625

anti-Bid antibody

anti-Bid antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody
nuts_pic
1

Overview

Product Description

Rabbit Polyclonal antibody recognizes Bid

Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Bid
Antigen Species Human
Immunogen Fusion protein of human Bid.
Conjugation Un-conjugated
Alternate Names p22 BID; BID; p15 BID; p11 BID; BH3-interacting domain death agonist; p13 BID; FP497

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P25-100
WB200-2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol
Preservative 0.05% Sodium azide
Stabilizer 40% Glycerol
Concentration 1.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 637 Human BID

Swiss-port # P55957 Human BH3-interacting domain death agonist

Gene Symbol BID
Gene Full Name BH3 interacting domain death agonist
Background This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
Highlight Related Antibody Duos and Panels:
ARG30275 Pro-apoptotic Bcl2 protein Antibody Panel (BAX, BAK, Bid)
Related products:
Bid antibodies; Bid Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody
Calculated MW 22 kDa
PTM TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.

Customer's Feedback

nuts_pic      Excellent

Review for anti-Bid antibody

Application:WB

Sample:Jurkat

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC