ARG22403

anti-Biotin antibody (FITC)

anti-Biotin antibody (FITC) for Flow cytometry,FLISA,Western blot and Other

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [SB58c] recognizes Biotin
Tested Reactivity Other
Tested Application FACS, FLISA, WB
Specificity The anti-Biotin antibody may be used for indirect immunostaining when working with a biotinylated primary antibody. As an effective alternative to avidin, Anti-Biotin amplifies signal and reduces background that can be caused by avidin and its derivatives.
Host Mouse
Clonality Monoclonal
Clone SB58c
Isotype IgG1
Target Name Biotin
Antigen Species Others
Immunogen KLH-conjugated Biotin
Conjugation FITC

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 1 µg/10^6 cells
FLISA1:200 - 1:400
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.