ARG62693
anti-Blood Group Lewis b antibody [2-25LE]
anti-Blood Group Lewis b antibody [2-25LE] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Mouse Monoclonal antibody [2-25LE] recognizes Blood Group Lewis b |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, WB |
| Specificity | The clone 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2-25LE |
| Isotype | IgG1 |
| Target Name | Blood Group Lewis b |
| Immunogen | Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification). |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Heat mediated antigen retrieval (sodium citrate); mAb incubated for 1 hour at RT; Detection DAB * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IHC-P: Colorectal adenocarcinoma |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Research Area | Cell Biology and Cellular Response antibody |
|---|
Clone References
Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses.
Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells.
Therapeutic targeting of Lewis(y) and Lewis(b) with a novel monoclonal antibody 692/29.
IHC-P, FACS / Human
Virus-induced appearance of the selectin ligand sLeX in herpes simplex virus type 1-infected T-cells: involvement of host and viral factors.
FACS / Human
Functional glycomic analysis of human milk glycans reveals the presence of virus receptors and embryonic stem cell biomarkers.
Presence of terminal N-acetylgalactosamineβ1-4N-acetylglucosamine residues on O-linked oligosaccharides from gastric MUC5AC: involvement in Helicobacter pylori colonization?
WB / Human
Investigation of unexpected serum CA19-9 elevation in Lewis-negative cancer patients.
