ARG56023

anti-Blood group A antigen antibody [HE-193]

anti-Blood group A antigen antibody [HE-193] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Mouse Monoclonal antibody [HE-193] recognizes Blood group A antigen
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P
Host Mouse
Clonality Monoclonal
Clone HE-193
Isotype IgM, kappa
Target Name Blood group A antigen
Antigen Species Human
Immunogen A mixture of erythrocytes of blood group A and glycoprotein fraction isolated from the saliva of secretors with blood group A.
Conjugation Un-conjugated
Alternate Names Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; Fucosylglycoprotein 3-alpha-galactosyltransferase; GTB; Histo-blood group A transferase; NAGAT; A3GALT1; Histo-blood group ABO system transferase; EC 2.4.1.40; A transferase; Histo-blood group B transferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; A3GALNT; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; B transferase; EC 2.4.1.37

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF0.5 - 1 µg/ml
IHC-P0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification PEG precipitation
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 28 Human ABO

Swiss-port # P16442 Human Histo-blood group ABO system transferase

Gene Symbol ABO
Gene Full Name ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)
Background This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene. [provided by RefSeq, Jul 2008]
Function This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. [UniProt]
Cellular Localization Cell surface
Calculated MW 41 kDa
PTM The soluble form derives from the membrane form by proteolytic processing.