ARG41381

anti-BubR1 antibody

anti-BubR1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Flow cytometry and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes BubR1
Tested Reactivity Hu
Tested Application FACS, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name BubR1
Antigen Species Human
Immunogen Synthetic peptide derived from Human BubR1.
Conjugation Un-conjugated
Alternate Names MVA1; Bub1A; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; SSK1; Mitotic checkpoint kinase MAD3L; Protein SSK1; hBUBR1; EC 2.7.11.1; MAD3L; BUBR1; BUB1beta

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
IHC-P1:50 - 1:200
IP1:30
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 120 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 701 Human BUB1B

Swiss-port # O60566 Human Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Gene Symbol BUB1B
Gene Full Name BUB1 mitotic checkpoint serine/threonine kinase B
Background This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008]
Function Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and KNL1. [UniProt]
Calculated MW 120 kDa
PTM Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.

Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.

Ubiquitinated. Degraded by the proteasome.

Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.

Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase. [UniProt]