ARG42103

anti-CA12 / Carbonic Anhydrase 12 antibody (Biotin)

anti-CA12 / Carbonic Anhydrase 12 antibody (Biotin) for Western blot and Human

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes CA12 / Carbonic Anhydrase 12
Tested Reactivity Hu
Tested Application WB
Specificity This antibody is expected to recognize all reported isoforms (NP_001209.1; NP_996808.1; NP_001280571.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name CA12 / Carbonic Anhydrase 12
Antigen Species Human
Immunogen Synthetic peptide around the internal region of Human CA12. (C-SHLQHVKYKGQE) (NP_001209.1; NP_996808.1; NP_001280571.1)
Conjugation Biotin
Alternate Names Carbonic anhydrase 12; Tumor antigen HOM-RCC-3.1.3; EC 4.2.1.1; HsT18816; Carbonic anhydrase XII; CAXII; CA-XII; Carbonate dehydratase XII

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human kidney
Observed Size ~ 42 kDa

Properties

Form Liquid
Purification Ammonium sulphate precipitation followed by affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 771 Human CA12

Swiss-port # O43570 Human Carbonic anhydrase 12

Gene Symbol CA12
Gene Full Name carbonic anhydrase XII
Background Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]
Function Reversible hydration of carbon dioxide. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 39 kDa