ARG43164

anti-CA2 / Carbonic Anhydrase 2 antibody

anti-CA2 / Carbonic Anhydrase 2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CA2 / Carbonic Anhydrase 2
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CA2 / Carbonic Anhydrase 2
Antigen Species Human
Immunogen Synthetic peptide derived from Human CA2 / Carbonic Anhydrase 2.
Conjugation Un-conjugated
Alternate Names Carbonic anhydrase C; EC 4.2.1.1; Car2; Carbonate dehydratase II; CA-II; Carbonic anhydrase II; CAC; HEL-76; Carbonic anhydrase 2; CAII

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 293T
Observed Size ~ 29 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide, 50% Glycerol and 0.05% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 760 Human CA2

Swiss-port # P00918 Human Carbonic anhydrase 2

Gene Symbol CA2
Gene Full Name carbonic anhydrase II
Background The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
Function Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6. [UniProt]
Cellular Localization Cytoplasm. Cell membrane. Note=Colocalized with SLC26A6 at the surface of the cell membrane in order to form a bicarbonate transport metabolon. Displaced from the cytosolic surface of the cell membrane by PKC in phorbol myristate acetate (PMA)-induced cells. [UniProt]
Calculated MW 29 kDa