ARG57300

anti-CA3 / Carbonic Anhydrase 3 antibody

anti-CA3 / Carbonic Anhydrase 3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CA3 / Carbonic Anhydrase 3
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CA3 / Carbonic Anhydrase 3
Antigen Species Human
Immunogen Recombinant Protein of Human CA3 / Carbonic Anhydrase 3.
Conjugation Un-conjugated
Alternate Names CAIII; EC 4.2.1.1; Car3; CA-III; Carbonate dehydratase III; Carbonic anhydrase 3; Carbonic anhydrase III

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse skeletal muscle

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 761 Human CA3

Swiss-port # P07451 Human Carbonic anhydrase 3

Gene Symbol CA3
Gene Full Name carbonic anhydrase III
Background Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]
Function Reversible hydration of carbon dioxide. [UniProt]
Calculated MW 30 kDa
PTM S-glutathionylated in hepatocytes under oxidative stress.