ARG57725

anti-CA9 / Carbonic Anhydrase 9 antibody [CA9/781]

anti-CA9 / Carbonic Anhydrase 9 antibody [CA9/781] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [CA9/781] recognizes CA9 / Carbonic Anhydrase 9
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone CA9/781
Isotype IgG2b, kappa
Target Name CA9 / Carbonic Anhydrase 9
Antigen Species Human
Immunogen Recombinant Human Carbonic Anhydrase 9 protein.
Conjugation Un-conjugated
Alternate Names EC 4.2.1.1; MN; CAIX; pMW1; Carbonic anhydrase 9; Carbonic anhydrase IX; RCC-associated antigen G250; Membrane antigen MN; P54/58N; Carbonate dehydratase IX; Renal cell carcinoma-associated antigen G250; CA-IX

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 - 5 µg/ml
WB0.5 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 50 kDa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 768 Human CA9

Swiss-port # Q16790 Human Carbonic anhydrase 9

Gene Symbol CA9
Gene Full Name carbonic anhydrase IX
Background Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
Function Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. [UniProt]
Calculated MW 50 kDa
PTM Asn-346 bears high-mannose type glycan structures. [UniProt]