ARG43591
anti-CACNA2D3 antibody
anti-CACNA2D3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Overview
Product Description | Rabbit Polyclonal antibody recognizes CACNA2D3 |
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Tested Reactivity | Ms, Rat |
Tested Application | IHC-P, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | CACNA2D3 |
Antigen Species | Rat |
Immunogen | Synthetic peptide derived from rat Cacna2d3. |
Conjugation | Un-conjugated |
Protein Full Name | Voltage-dependent calcium channel subunit alpha-2/delta-3 |
Alternate Names | HSA272268; Voltage-gated calcium channel subunit alpha-2/delta-3; Voltage-dependent calcium channel subunit alpha-2-3; Voltage-dependent calcium channel subunit delta-3 |
Application Instructions
Application Suggestion |
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Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Tris-EDTA pH9.0 for 20 min. Incubate with antibody at RT for 30-60 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Positive Control | Mouse brain |
Properties
Form | Powder |
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Purification | Unpurified |
Buffer | Whole serum |
Reconstitution | Reconstitute in 100 μl of sterile water. When reconstituting 0.1% sodium azide or 1:1 (v/v) glycerol may be added for an additional stability. |
Storage Instruction | Storage lyophilised/reconstituted antibody at -20°C. After reconstitution, for continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store reconstituted antibody at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # Q8CFG5 Rat Voltage-dependent calcium channel subunit alpha-2/delta-3 Swiss-port # Q9Z1L5 Mouse Voltage-dependent calcium channel subunit alpha-2/delta-3 |
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Gene Symbol | CACNA2D3 |
Gene Full Name | calcium voltage-gated channel auxiliary subunit alpha2delta 3 |
Background | CACNA2D3 gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
Function | The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) but not T-type (CACNA1G) (By similarity). |
Cellular Localization | Membrane |
Calculated MW | 122 kDa |
PTM | Disulfide bond, Glycoprotein, Phosphoprotein N-glycosylated May be proteolytically processed into subunits alpha-2-3 and delta-3 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role (By similarity). |