ARG43591

anti-CACNA2D3 antibody

anti-CACNA2D3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CACNA2D3
Tested Reactivity Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CACNA2D3
Antigen Species Rat
Immunogen Synthetic peptide derived from rat Cacna2d3.
Conjugation Un-conjugated
Protein Full Name Voltage-dependent calcium channel subunit alpha-2/delta-3
Alternate Names HSA272268; Voltage-gated calcium channel subunit alpha-2/delta-3; Voltage-dependent calcium channel subunit alpha-2-3; Voltage-dependent calcium channel subunit delta-3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:1000
WB1:1000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Tris-EDTA pH9.0 for 20 min. Incubate with antibody at RT for 30-60 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse brain

Properties

Form Powder
Purification Unpurified
Buffer Whole serum
Reconstitution Reconstitute in 100 μl of sterile water. When reconstituting 0.1% sodium azide or 1:1 (v/v) glycerol may be added for an additional stability.
Storage Instruction Storage lyophilised/reconstituted antibody at -20°C. After reconstitution, for continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store reconstituted antibody at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12294 Mouse CACNA2D3

GeneID: 306243 Rat CACNA2D3

Swiss-port # Q8CFG5 Rat Voltage-dependent calcium channel subunit alpha-2/delta-3

Swiss-port # Q9Z1L5 Mouse Voltage-dependent calcium channel subunit alpha-2/delta-3

Gene Symbol CACNA2D3
Gene Full Name calcium voltage-gated channel auxiliary subunit alpha2delta 3
Background CACNA2D3 gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
Function The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) but not T-type (CACNA1G) (By similarity).
Cellular Localization Membrane
Calculated MW 122 kDa
PTM Disulfide bond, Glycoprotein, Phosphoprotein N-glycosylated May be proteolytically processed into subunits alpha-2-3 and delta-3 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role (By similarity).