ARG55410

anti-CARD11 antibody

anti-CARD11 antibody for Western blot,Immunohistochemistry,ICC/IF and Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CARD11
Tested Reactivity Ms
Predict Reactivity Hu
Tested Application ICC/IF, IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CARD11
Antigen Species Human
Immunogen Synthetic peptide within the last 50 aa of Human CARD11.
Conjugation Un-conjugated
Alternate Names BIMP3; BENTA; PPBL; Caspase recruitment domain-containing protein 11; Carma 1; CARD-containing MAGUK protein 1; IMD11; CARMA1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF20 μg/ml
IHC10 μg/ml
WB2 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse Thymus Tissue Lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 108723 Mouse CARD11

Swiss-port # Q8CIS0 Mouse Caspase recruitment domain-containing protein 11

Gene Symbol CARD11
Gene Full Name caspase recruitment domain family, member 11
Background The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008]
Function Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 133 kDa
PTM Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCB and PRKCQ leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.