ARG58342

anti-CARS / CysRS antibody

anti-CARS / CysRS antibody for ICC/IF,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes CARS / CysRS
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CARS / CysRS
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 469-748 of Human CARS (NP_001742.1).
Conjugation Un-conjugated
Alternate Names Cysteine--tRNA ligase, cytoplasmic; Cysteinyl-tRNA synthetase; CYSRS; MGC:11246; EC 6.1.1.16; CARS1; CysRS

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size 85 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 27267 Mouse CARS

GeneID: 833 Human CARS

Swiss-port # P49589 Human Cysteine--tRNA ligase, cytoplasmic

Swiss-port # Q9ER72 Mouse Cysteine--tRNA ligase, cytoplasmic

Gene Symbol CARS
Gene Full Name cysteinyl-tRNA synthetase
Background This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 85 kDa