ARG53997
anti-CBX3 / HP1 gamma antibody
anti-CBX3 / HP1 gamma antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Hamster,Human,Monkey,Mouse,Rat
Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes CBX3 / HP1 gamma |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Hm, Mk |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | CBX3 / HP1 gamma |
| Antigen Species | Human |
| Immunogen | Purified recombinant Human HP1 gamma protein fragments. |
| Conjugation | Un-conjugated |
| Alternate Names | HP1-GAMMA; Chromobox protein homolog 3; HP1Hs-gamma; HP1 gamma; HECH; Heterochromatin protein 1 homolog gamma; Modifier 2 protein |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 22 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 0.75 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CBX3 |
| Gene Full Name | chromobox homolog 3 |
| Background | Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. |
| Function | Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the mehtyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. [UniProt] |
| Cellular Localization | Nucleus |
| Highlight | Related Antibody Duos and Panels: ARG30341 Heterochromatin Foci Antibody Duo Related products: CBX3 antibodies; CBX3 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Senescence Marker Antibody Panel is launched |
| Research Area | Gene Regulation antibody |
| Calculated MW | 21 kDa |
| PTM | Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. |
