ARG65986

anti-CCL16 / LEC antibody [1.2_5G3-1D7]

anti-CCL16 / LEC antibody [1.2_5G3-1D7] for ELISA,Neutralizing,Western blot and Human

Overview

Product Description

Mouse Monoclonal antibody [1.2_5G3-1D7] recognizes CCL16 / LEC

Tested Reactivity Hu
Tested Application ELISA, Neut, WB
Host Mouse
Clonality Monoclonal
Clone 1.2_5G3-1D7
Isotype IgG1, kappa
Target Name CCL16 / LEC
Antigen Species Human
Immunogen E. coli derived recombinant Human CCL16 / LEC.
(QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNSQ)
Conjugation Un-conjugated
Alternate Names CKb12; HCC-4; LEC; Monotactin-1; SCYA16; Chemokine LEC; MTN-1; SCYL4; Mtn-1; Lymphocyte and monocyte chemoattractant; Small-inducible cytokine A16; Liver-expressed chemokine; IL-10-inducible chemokine; C-C motif chemokine 16; NCC4; LCC-1; ILINCK; LMC; Chemokine CC-4; NCC-4

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 2.0 - 4.0 µg/ml combined with a detection antibody
Neut1.0 - 5.0 µg/ml (To yield [ND50] of the biological activity of Human LEC (100 ng/ml) )
WB0.20 - 0.40 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6360 Human CCL16

Swiss-port # O15467 Human C-C motif chemokine 16

Gene Symbol CCL16
Gene Full Name chemokine (C-C motif) ligand 16
Background This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. This cytokine also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. The expression of this gene is upregulated by IL-10. [provided by RefSeq, Jul 2008]
Function Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES. [UniProt]
Calculated MW 14 kDa