ARG56590

anti-CCL2 / MCP1 antibody [2.2-4H5-1A11]

anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Cow,Human,Mouse

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Overview

Product Description Mouse Monoclonal antibody [2.2-4H5-1A11] recognizes CCL2 / MCP1
Tested Reactivity Hu, Ms, Cow
Tested Application ELISA, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 2.2-4H5-1A11
Isotype IgG1, kappa
Target Name CCL2 / MCP1
Antigen Species Human
Immunogen E.coli derived Recombinant Human CCL2 / MCP1.
(QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT)
Conjugation Un-conjugated
Alternate Names MCP1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; HSMCR30; Small-inducible cytokine A2; HC11; SMC-CF; GDCF-2; SCYA2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 2.0 - 4.0 µg/ml with ARG56731 as a detection antibody
IHC-P10.0 µg/ml
WB0.20 - 0.40 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20296 Mouse CCL2

GeneID: 6347 Human CCL2

Swiss-port # P10148 Mouse C-C motif chemokine 2

Swiss-port # P13500 Human C-C motif chemokine 2

Gene Symbol CCL2
Gene Full Name chemokine (C-C motif) ligand 2
Background This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
Function Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. [UniProt]
Highlight Related products:
MCP1 antibodies; MCP1 ELISA Kits; MCP1 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Calculated MW 11 kDa (Human); 16 kDa (Mouse)
PTM Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

Specific References

Exosomal miR-7002-5p derived from highglucose-induced macrophages suppresses autophagy in tubular epithelial cells by targeting Atg9b

IHC-P / Mouse

Jing Zhao  et al.
FASEB J.,  (2022)

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Ramulus Mori (Sangzhi) Alkaloids (SZ-A) Ameliorate Glucose Metabolism Accompanied by the Modulation of Gut Microbiota and Ileal Inflammatory Damage in Type 2 Diabetic KKAy Mice.

WB / Mouse

Quan Liu et al.
Front Pharmacol.,  (2021)

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