ARG54724

anti-CCL2 / MCP1 antibody

anti-CCL2 / MCP1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CCL2 / MCP1
Tested Reactivity Hu
Predict Reactivity Mk
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name CCL2 / MCP1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 62-89 (C-terminus) of Human CCL2 (NP_002973.1).
Conjugation Un-conjugated
Alternate Names MCP1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; HSMCR30; Small-inducible cytokine A2; HC11; SMC-CF; GDCF-2; SCYA2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa

Properties

Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6347 Human CCL2

Swiss-port # P13500 Human C-C motif chemokine 2

Gene Symbol CCL2
Gene Full Name chemokine (C-C motif) ligand 2
Background This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
Function Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. [From Uniprot]
Cellular Localization Secreted.
Highlight Related products:
MCP1 antibodies; MCP1 ELISA Kits; MCP1 Duos / Panels;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody
Calculated MW 11 kDa
PTM Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.