ARG56650

anti-CCL3 / MIP1 alpha antibody

anti-CCL3 / MIP1 alpha antibody for ELISA,Western blot,Neutralizing and Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CCL3 / MIP1 alpha
Tested Reactivity Rat
Tested Application ELISA, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CCL3 / MIP1 alpha
Antigen Species Rat
Immunogen E.coli derived Recombinant Rat MIP-1α (CCL3).
(APYGADTPTA CCFSYGRQIP RKFIADYFET SSLCSQPGVI FLTKRNRQIC ADPKETWVQE YITELELNA)
Conjugation Un-conjugated
Alternate Names MIP-1-alpha; SIS-beta; PAT 464.1; Tonsillar lymphocyte LD78 alpha protein; G0/G1 switch regulatory protein 19-1; Small-inducible cytokine A3; C-C motif chemokine 3; LD78-alpha; 4-69; Macrophage inflammatory protein 1-alpha; MIP1A; SCYA3; G0S19-1; LD78ALPHA

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56759 as a detection antibody
Neut8.0 - 10.0 µg/ml (To yield [ND50] of the biological activity of Rat MIP - 1α (100 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25542 Rat CCL3

Swiss-port # P50229 Rat C-C motif chemokine 3

Gene Symbol Ccl3
Gene Full Name chemokine (C-C motif) ligand 3
Background This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]
Function Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). [UniProt]
Calculated MW 10 kDa
PTM N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.