ARG59022

anti-CCN3 antibody

anti-CCN3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry,ICC/IF and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CCN3
Tested Reactivity Hu, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CCN3
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 334-357 of Human CCN3 (HTNCPKNNEAFLQELELKTTRGKM).
Conjugation Un-conjugated
Alternate Names Insulin-like growth factor-binding protein 9; Protein NOV homolog; IGFBP-9; NOVh; IBP-9; CCN3; Nephroblastoma-overexpressed gene protein homolog; CCN family member 3; IGF-binding protein 9; IGFBP9; NovH

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4856 Human NOV

GeneID: 81526 Rat NOV

Swiss-port # P48745 Human Protein NOV homolog

Swiss-port # Q9QZQ5 Rat Protein NOV homolog

Gene Symbol NOV
Gene Full Name nephroblastoma overexpressed
Background The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]
Function Immediate-early protein likely to play a role in cell growth regulation. [UniProt]
Cellular Localization Secreted. [UniProt]
Calculated MW 39 kDa