ARG23532
anti-CD101 antibody [BB27] (PE)
anti-CD101 antibody [BB27] (PE) for Flow cytometry and Human
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [BB27] recognizes CD101. Mouse anti Human CD101 antibody, clone BB27 recognizes human CD101. CD101 is a ~140 kDa homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes. CD101 plays a major role in the activation of T cells by skin dendritic cells. Mouse anti Human CD101 antibody, clone BB27 has been reported to inhibit allogeneic T-cell responses (Bagot et al. 1997). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | BB27 |
| Isotype | IgG1 |
| Target Name | CD101 |
| Antigen Species | Human |
| Immunogen | Human thymic clone B12. |
| Conjugation | PE |
| Alternate Names | Immunoglobulin superfamily member 2; IgSF2; CD antigen CD101; Cell surface glycoprotein V7; EWI-101; IGSF2; V7; Glu-Trp-Ile EWI motif-containing protein 101 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA and 5% Sucrose |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q93033 Human Immunoglobulin superfamily member 2 |
|---|---|
| Gene Symbol | CD101 |
| Gene Full Name | CD101 molecule |
| Function | Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells. May be a marker of CD4(+) CD56(+) leukemic tumor cells. [UniProt] |
| Calculated MW | 115 kDa |
| PTM | N-glycosylated. [UniProt] |
