ARG54221
anti-CD101 antibody [BB27] (PE)
anti-CD101 antibody [BB27] (PE) for Flow cytometry and Human
Immune System antibody
Overview
Product Description | PE-conjugated Mouse Monoclonal antibody [BB27] recognizes CD101 |
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Tested Reactivity | Hu |
Tested Application | FACS |
Specificity | The clone BB27 recognizes CD101, a 140 kDa disulfide-bonded homodimeric protein expressed on activated T cells, and some ther cell types, such as granulocytes and cells of the monocyte/macropgage lineage. HLDA V; WS Code T040 |
Host | Mouse |
Clonality | Monoclonal |
Clone | BB27 |
Isotype | IgG1 |
Target Name | CD101 |
Antigen Species | Human |
Immunogen | Human thymic clone B12 |
Conjugation | PE |
Alternate Names | Immunoglobulin superfamily member 2; IgSF2; CD antigen CD101; Cell surface glycoprotein V7; EWI-101; IGSF2; V7; Glu-Trp-Ile EWI motif-containing protein 101 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
Preservative | 15 mM Sodium azide |
Stabilizer | 0.2% (w/v) high-grade protease free BSA |
Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # Q93033 Human Immunoglobulin superfamily member 2 |
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Gene Symbol | CD101 |
Gene Full Name | CD101 molecule |
Background | CD101 is a type I transmembrane glycoprotein, which forms disulfide-linked homodimers. It is expressed on activated T cells, as well as on granulocytes, monocytes, dendritic cells or mucosal T cells. It plays a major role in the activation of T cells by skin dendritic cells. Function of CD101 has not been fully elucidated, but in mice its knock-out results in liver autoimmune disease induced by Novosphingobium aromaticivorans. |
Function | Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells. May be a marker of CD4(+) CD56(+) leukemic tumor cells. [UniProt] |
Research Area | Immune System antibody |
Calculated MW | 115 kDa |
PTM | N-glycosylated. |