ARG54226
anti-CD103 / Integrin alpha E antibody [Ber-ACT8] (APC)
anti-CD103 / Integrin alpha E antibody [Ber-ACT8] (APC) for Flow cytometry and Human,Primates
Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | APC-conjugated Mouse Monoclonal antibody [Ber-ACT8] recognizes CD103 / Integrin alpha E |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS |
| Specificity | The clone Ber-ACT8 recognizes CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes. HLDA V; WS Code A067 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | Ber-ACT8 |
| Isotype | IgG1 |
| Target Name | CD103 / Integrin alpha E |
| Antigen Species | Human |
| Immunogen | HTLV-1 induced human T cell line MAPS16 |
| Conjugation | APC |
| Alternate Names | CD103; HUMINAE; Integrin alpha-E; Mucosal lymphocyte 1 antigen; CD antigen CD103; HML-1 antigen; Integrin alpha-IEL |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | ITGAE |
| Gene Full Name | integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) |
| Background | CD103 / Integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation. |
| Function | Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 130 kDa |
