ARG23419

anti-CD104 / Integrin beta 4 antibody [450-9D]

anti-CD104 / Integrin beta 4 antibody [450-9D] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Ferret,Human

Overview

Product Description Mouse Monoclonal antibody [450-9D] recognizes CD104 / Integrin beta 4
Mouse anti Human CD104 antibody, clone 450-9D recognizes the human beta4 integrin, also known as CD104. CD104 is a ~205 kDa glycoprotein which associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumor cell lines. Mouse anti Human CD104 antibody, clone 450-9D recognizes an extracellular epitope on the CD104 molecule.
Tested Reactivity Hu, Frt
Tested Application FACS, ICC/IF, IHC-Fr, IP, WB
Host Mouse
Clonality Monoclonal
Clone 450-9D
Isotype IgG1
Target Name CD104 / Integrin beta 4
Antigen Species Human
Immunogen Purified alpha6 beta4 integrin from A431 cells.
Conjugation Un-conjugated
Alternate Names Integrin beta-4; GP150; CD antigen CD104; CD104

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:25 - 1:100
ICC/IFAssay-dependent
IHC-Fr1:500 - 1:1000
IPAssay-dependent
WBAssay-dependent
Application Note IHC-Fr: The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3691 Human ITGB4

Swiss-port # P16144 Human Integrin beta-4

Gene Symbol ITGB4
Gene Full Name integrin, beta 4
Background Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. [UniProt]
Calculated MW 202 kDa
PTM Palmitoylated by DHHC3 at several cysteines of the membrane-proximal region, enhancing stability and cell surface expression. Palmitoylation also promotes secundary association with tertaspanins. [UniProt]

Clone References

Surface Immobilization Chemistry of a Laminin-Derived Peptide Affects Keratinocyte Activity

IHC-Fr / Human

Nicholas G Fischer et al.
Coatings (Basel).,  (2020)

publication_link

 

hr_line

Loss of β-PIX inhibits focal adhesion disassembly and promotes keratinocyte motility via myosin light chain activation

ICC/IF / Human

Sho Hiroyasu et al.
J Cell Sci.,  (2017)

publication_link

 

hr_line

Expression of laminin isoforms, receptors, and binding proteins unique to nucleus pulposus cells of immature intervertebral disc

IHC-Fr / Human

Jun Chen et al.
Connect Tissue Res.,  (2009)

publication_link

 

hr_line

Loss of keratins 8 and 18 leads to alterations in α6β4-integrin-mediated signalling and decreased neoplastic progression in an oral-tumour-derived cell line

FACS / Human

Hunain Alam et al.
J Cell Sci.,  (2011)

publication_link

 

hr_line

TGF-β-Induced Quiescence Mediates Chemoresistance of Tumor-Propagating Cells in Squamous Cell Carcinoma

IHC-Fr / Human

Jessie A Brown et al.
Cell Stem Cell.,  (2017)

publication_link

 

hr_line

Reconstruction of a human hemicornea through natural scaffolds compatible with the growth of corneal epithelial stem cells and stromal keratocytes

ICC/IF / Human

Vanessa Barbaro et al.
Mol Vis.,  (2009)

publication_link

 

hr_line