ARG62699

anti-CD105 / Endoglin antibody [MEM-226]

anti-CD105 / Endoglin antibody [MEM-226] for CyTOF®-candidate,Flow cytometry,Immunoprecipitation,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody
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Overview

Product Description

Mouse Monoclonal antibody [MEM-226] recognizes CD105 / Endoglin

Tested Reactivity Hu
Tested Application CyTOF®-candidate, FACS, IP, WB
Specificity The clone MEM-226 reacts with CD105 (Endoglin), a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.
Host Mouse
Clonality Monoclonal
Clone MEM-226
Isotype IgG2a
Target Name CD105 / Endoglin
Antigen Species Human
Immunogen Recombinant Vaccinia virus containing the human CD105 cDNA.
Conjugation Un-conjugated
Alternate Names CD antigen CD105; HHT1; Endoglin; ORW1; END

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS2 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2022 Human ENG

Swiss-port # P17813 Human Endoglin

Gene Symbol ENG
Gene Full Name endoglin
Background CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
Function Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody
Calculated MW 71 kDa

Customer's Feedback

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Review for anti-CD105 / Endoglin antibody [MEM-226]

Application:WB

Sample:HUVEC

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Clone References

Conversion of human fibroblasts to angioblast-like progenitor cells.

Kurian L et al.
Nat Methods.,  (2013)

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An integrated transcriptional regulatory circuit that reinforces the breast cancer stem cell state.

Polytarchou C et al.
Proc Natl Acad Sci U S A.,  (2012)

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Age-related changes in rat bone-marrow mesenchymal stem cell plasticity.

FACS / Rat

Asumda FZ et al.
BMC Cell Biol.,  (2011)

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Human ovarian carcinoma–associated mesenchymal stem cells regulate cancer stem cells and tumorigenesis via altered BMP production.

FACS / Human

McLean K et al.
J Clin Invest.,  (2011)

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Inducible formation of breast cancer stem cells and their dynamic equilibrium with non-stem cancer cells via IL6 secretion.

Puri / Human

Iliopoulos D et al.
Proc Natl Acad Sci U S A.,  (2011)

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The effects of 17-β estradiol on enhancing proliferation of human bone marrow mesenchymal stromal cells in vitro.

FACS / Human

Hong L et al.
Stem Cells Dev.,  (2011)

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