ARG62700
anti-CD105 / Endoglin antibody [MEM-229]
anti-CD105 / Endoglin antibody [MEM-229] for Flow cytometry,ICC/IF,IHC-Frozen sections,Western blot and Human,Pig
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-229] recognizes CD105 / Endoglin |
|---|---|
| Tested Reactivity | Hu, Pig |
| Tested Application | FACS, ICC/IF, IHC-Fr, WB |
| Specificity | The clone MEM-229 recognizes CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-229 |
| Isotype | IgG2a |
| Target Name | CD105 / Endoglin |
| Antigen Species | Human |
| Immunogen | Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA. |
| Conjugation | Un-conjugated |
| Alternate Names | CD antigen CD105; HHT1; Endoglin; ORW1; END |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Under non-reducing condition. IHC-Fr: Acetone fixation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | FACS: Kg1 human acute myelogenous leukemia cell line |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ENG |
| Gene Full Name | endoglin |
| Background | CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels. |
| Function | Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody |
| Calculated MW | 71 kDa |
Clone References
Disruption of endothelial adherens junctions by high glucose is mediated by protein kinase C-β-dependent vascular endothelial cadherin tyrosine phosphorylation.
A fat option for the pig: hepatocytic differentiated mesenchymal stem cells for translational research.
FACS / Pig
CD200 positive human mesenchymal stem cells suppress TNF-alpha secretion from CD200 receptor positive macrophage-like cells.
FACS / Human
Mitochondrial function and energy metabolism in umbilical cord blood- and bone marrow-derived mesenchymal stem cells.
FACS / Human
Tumor necrosis factor alpha promotes the expression of immunosuppressive proteins and enhances the cell growth in a human bone marrow-derived stem cell culture.
FACS / Human
Gadonanotubes as magnetic nanolabels for stem cell detection.
FACS / Pig
