ARG23261
anti-CD106 / VCAM1 antibody [MVCAM A (429)]
anti-CD106 / VCAM1 antibody [MVCAM A (429)] for IHC-Frozen sections,Flow cytometry and Mouse
Overview
| Product Description | Rat Monoclonal antibody [MVCAM A (429)] recognizes CD106 / VCAM1 Rat anti Mouse CD106 antibody, clone MVCAM A (429) recognizes murine vascular adhesion molecule 1 (VCAM-1), a cell surface glycoprotein that is also known as CD106. CD106 is expressed predominantly on endothelial cells and expression is up-regulated during inflammation. The ligand for CD106 is the alpha 4 subunit (CD49d) of the integrin VLA-4 (CD49d/CD29). Rat anti Mouse CD106 antibody, clone MVCAM A (429) is reported to partially block VCAM-1 mediated functions (Kinashi and Springer 1994). |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, IHC-Fr |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | MVCAM A (429) |
| Isotype | IgG2a |
| Target Name | CD106 / VCAM1 |
| Antigen Species | Mouse |
| Immunogen | Stromal cell line PA6. |
| Conjugation | Un-conjugated |
| Alternate Names | CD106; INCAM-100; Vascular cell adhesion protein 1; VCAM-1; CD antigen CD106; V-CAM 1 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | VCAM1 |
| Gene Full Name | vascular cell adhesion molecule 1 |
| Background | This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010] |
| Function | Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. [UniProt] |
| Calculated MW | 81 kDa |
| PTM | Sialoglycoprotein. [UniProt] |
