ARG65453

anti-CD107a / LAMP1 antibody [H4A3] (azide free)

anti-CD107a / LAMP1 antibody [H4A3] (azide free) for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Primates,Rat

Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody
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Overview

Product Description Azide free Mouse Monoclonal antibody [H4A3] recognizes CD107a / LAMP1
Tested Reactivity Hu, Ms, Rat, NHuPrm
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The mouse monoclonal antibody H4A3 recognizes LAMP1 / CD107a, an approximately 100120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.
Host Mouse
Clonality Monoclonal
Clone H4A3
Isotype IgG1
Target Name CD107a / LAMP1
Antigen Species Human
Immunogen Human PBMC
Conjugation Un-conjugated
Alternate Names LGP120; CD107a; LAMPA; CD antigen CD107a; Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; LAMP-1; Lysosome-associated membrane protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WB1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer BBS (pH 8.2)
Concentration 0.1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16783 Mouse LAMP1

GeneID: 25328 Rat LAMP1

GeneID: 3916 Human LAMP1

Gene Symbol LAMP1
Gene Full Name lysosomal-associated membrane protein 1
Background CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
Function Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. [UniProt]
Highlight Related products:
LAMP1 antibodies; LAMP1 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
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Research Area Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody
Calculated MW 45 kDa
PTM O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells.

Customer's Feedback

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Review for anti-CD107a / LAMP1 antibody [H4A3] (azide free)

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:50

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Excellent

Review for anti-CD107a / LAMP1 antibody [H4A3] (azide free)

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Clone References

Cellular mechanisms of toll-like receptor-3 activation in the thalamus are associated with white matter injury in the developing brain.

Vontell R et al.
J Neuropathol Exp Neurol.,  (2015)

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The role of the helper lipid on the DNA transfection efficiency of lipopolyplex formulations.

Du Z et al.
Sci Rep.,  (2014)

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Induced pluripotent stem cells restore function in a human cell loss model of open-angle glaucoma.

Abu-Hassan DW et al.
Stem Cells.,  (2015)

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Glutamine deprivation stimulates mTOR-JNK-dependent chemokine secretion.

ICC/IF / Human

Shanware NP et al.
Nat Commun.,  (2014)

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QSOX1 inhibits autophagic flux in breast cancer cells.

ICC/IF / Human

Poillet L et al.
PLoS One.,  (2014)

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Filamin A promotes dynamin-dependent internalization of hyperpolarization-activated cyclic nucleotide-gated type 1 (HCN1) channels and restricts Ih in hippocampal neurons.

ICC/IF / Rat

Noam Y et al.
J Biol Chem.,  (2014)

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Blood-brain barrier impairment in MPS III patients.

IHC-Fr / Human

Garbuzova-Davis S et al.
BMC Neurol.,  (2013)

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Pathogenic Parkinson's disease mutations across the functional domains of LRRK2 alter the autophagic/lysosomal response to starvation.

WB / Human

Manzoni C et al.
Biochem Biophys Res Commun.,  (2013)

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Autophagy in retinal ganglion cells in a rhesus monkey chronic hypertensive glaucoma model.

WB / Rhesus monkey

Deng S et al.
PLoS One.,  (2013)

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Hypertonic stress increases claudin-4 expression and tight junction integrity in association with MUPP1 in IMCD3 cells.

ICC/IF / Mouse

Lanaspa MA et al.
Proc Natl Acad Sci U S A.,  (2008)

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