ARG65453
anti-CD107a / LAMP1 antibody [H4A3] (azide free)
anti-CD107a / LAMP1 antibody [H4A3] (azide free) for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Primates,Rat
Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody
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Overview
Product Description | Azide free Mouse Monoclonal antibody [H4A3] recognizes CD107a / LAMP1 |
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Tested Reactivity | Hu, Ms, Rat, NHuPrm |
Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
Specificity | The mouse monoclonal antibody H4A3 recognizes LAMP1 / CD107a, an approximately 100120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane. |
Host | Mouse |
Clonality | Monoclonal |
Clone | H4A3 |
Isotype | IgG1 |
Target Name | CD107a / LAMP1 |
Antigen Species | Human |
Immunogen | Human PBMC |
Conjugation | Un-conjugated |
Alternate Names | LGP120; CD107a; LAMPA; CD antigen CD107a; Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; LAMP-1; Lysosome-associated membrane protein 1 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Buffer | BBS (pH 8.2) |
Concentration | 0.1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | LAMP1 |
Gene Full Name | lysosomal-associated membrane protein 1 |
Background | CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation. |
Function | Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. [UniProt] |
Highlight | Related products: LAMP1 antibodies; LAMP1 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Baking soda restores circadian clock in tumor cells |
Research Area | Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody |
Calculated MW | 45 kDa |
PTM | O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells. |
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Review for anti-CD107a / LAMP1 antibody [H4A3] (azide free)
Application:IF/ICC
Sample:HeLa
Fixation Buffer:100% Methanol
Fixation Time:10 min
Fixation Temperature:RT ºC
Permeabilization Buffer:0.1% Triton X-100
Primary Antibody Dilution Factor:1:50
Primary Antibody Incubation Time:overnight
Primary Antibody Incubation Temperature:4 ºC
Conjugation of Secondary Antibody:FITC
Clone References