ARG54627

anti-CD107a / LAMP1 antibody [LY1C6]

anti-CD107a / LAMP1 antibody [LY1C6] for ICC/IF,Immunoprecipitation,Western blot and Hamster,Rat

Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody

Overview

Product Description Mouse Monoclonal antibody [LY1C6] recognizes CD107a / LAMP1
Tested Reactivity Rat, Hm
Tested Application ICC/IF, IP, WB
Specificity This antibody recognizes LAMP1 of rat andhamster.
Host Mouse
Clonality Monoclonal
Clone LY1C6
Isotype IgG1
Target Name CD107a / LAMP1
Antigen Species Rat
Immunogen Rat liver lysosome membranes
Conjugation Un-conjugated
Alternate Names LGP120; CD107a; LAMPA; CD antigen CD107a; Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; LAMP-1; Lysosome-associated membrane protein 1

Application Instructions

Application Note Western blot: use at 1 ug/ml. A band of ~120 kDa is detected.
Immunocytochemistry: use at 5 - 10 ug/ml.
Immunoprecipitation: use at 5 ug/ml
Affinity purification of LAMP1: use at 2 - 4mg/ml of CnBR - activated Sepharose 4B.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat liver microsomes

Properties

Form Liquid
Purification Protein G affinity chromatography
Buffer PBS (pH 7.4), 50% Glycerol and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25328 Rat LAMP1

Swiss-port # P14562 Rat Lysosome-associated membrane glycoprotein 1

Gene Symbol Lamp1
Gene Full Name lysosomal-associated membrane protein 1
Background Lysosome-associated membrane proteins(LAMP1 and LAMP2) are major constituentsof the lysosomal membrane. These twoproteins have closely related structures with37% sequence homology. Both aretransmembrane glycoproteins localizedprimarily in lysosomes and late endosomes.Upon cell activation, rapid translocation ofintracellular LAMPs to the cell membrane isdependent on a carboxyl-terminal tyrosinebasedmotif (YXXI). Disruption of thespacing between the tyrosine-based motifabolishes lysosome localization of LAMP1,and the altered protein cycles between themembrane and the endosome. Cell surfaceLAMP1 and LAMP2 promote adhesion ofhuman peripheral blood mononuclear cells(PBMC) to vascular endothelium whichsuggests that they are involved in adhesionof PBMC at sites of inflammation.
Function Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. [UniProt]
Research Area Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody
Calculated MW 45 kDa
PTM O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells.

Clone References

Subcellular localization of talin is regulated by inter-domain interactions.

WB / 

Banno A et al.
J Biol Chem.,  (2012)

publication_link

 

hr_line

In vitro motility of liver connexin vesicles along microtubules utilizes kinesin motors.

ICC/IF / Rat

Fort AG et al.
J Biol Chem.,  (2011)

publication_link

 

hr_line

Hypertonic stress increases claudin-4 expression and tight junction integrity in association with MUPP1 in IMCD3 cells.

ICC/IF / Mouse

Lanaspa MA et al.
Proc Natl Acad Sci U S A.,  (2008)

publication_link

 

hr_line

Route of uptake of palmitoylated encephalitogenic peptides of myelin proteolipid protein by antigen-presenting cells: importance of the type of bond between lipid chain and peptide and relevance to autoimmunity.

ICC/IF / Mouse

Pfender NA et al.
J Immunol.,  (2008)

publication_link

 

hr_line

Neurotrophins Redirect p75NTR from a clathrin-independent to a clathrin-dependent endocytic pathway coupled to axonal transport.

ICC/IF / Rat

Deinhardt K et al.
Traffic.,  (2007)

publication_link

 

hr_line

Sorafenib functions to potently suppress RET tyrosine kinase activity by direct enzymatic inhibition and promoting RET lysosomal degradation independent of proteasomal targeting.

ICC/IF / Human

Plaza-Menacho I et al.
J Biol Chem.,  (2007)

publication_link

 

hr_line