ARG54627
anti-CD107a / LAMP1 antibody [LY1C6]
anti-CD107a / LAMP1 antibody [LY1C6] for ICC/IF,Immunoprecipitation,Western blot and Hamster,Rat
Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [LY1C6] recognizes CD107a / LAMP1 |
|---|---|
| Tested Reactivity | Rat, Hm |
| Tested Application | ICC/IF, IP, WB |
| Specificity | This antibody recognizes LAMP1 of rat andhamster. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | LY1C6 |
| Isotype | IgG1 |
| Target Name | CD107a / LAMP1 |
| Antigen Species | Rat |
| Immunogen | Rat liver lysosome membranes |
| Conjugation | Un-conjugated |
| Alternate Names | LGP120; CD107a; LAMPA; CD antigen CD107a; Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; LAMP-1; Lysosome-associated membrane protein 1 |
Application Instructions
| Application Note | Western blot: use at 1 ug/ml. A band of ~120 kDa is detected. Immunocytochemistry: use at 5 - 10 ug/ml. Immunoprecipitation: use at 5 ug/ml Affinity purification of LAMP1: use at 2 - 4mg/ml of CnBR - activated Sepharose 4B. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|---|
| Positive Control | Rat liver microsomes |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity chromatography |
| Buffer | PBS (pH 7.4), 50% Glycerol and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P14562 Rat Lysosome-associated membrane glycoprotein 1 |
|---|---|
| Gene Symbol | Lamp1 |
| Gene Full Name | lysosomal-associated membrane protein 1 |
| Background | Lysosome-associated membrane proteins(LAMP1 and LAMP2) are major constituentsof the lysosomal membrane. These twoproteins have closely related structures with37% sequence homology. Both aretransmembrane glycoproteins localizedprimarily in lysosomes and late endosomes.Upon cell activation, rapid translocation ofintracellular LAMPs to the cell membrane isdependent on a carboxyl-terminal tyrosinebasedmotif (YXXI). Disruption of thespacing between the tyrosine-based motifabolishes lysosome localization of LAMP1,and the altered protein cycles between themembrane and the endosome. Cell surfaceLAMP1 and LAMP2 promote adhesion ofhuman peripheral blood mononuclear cells(PBMC) to vascular endothelium whichsuggests that they are involved in adhesionof PBMC at sites of inflammation. |
| Function | Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. [UniProt] |
| Research Area | Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody |
| Calculated MW | 45 kDa |
| PTM | O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells. |
Clone References
Subcellular localization of talin is regulated by inter-domain interactions.
WB /
In vitro motility of liver connexin vesicles along microtubules utilizes kinesin motors.
ICC/IF / Rat
Hypertonic stress increases claudin-4 expression and tight junction integrity in association with MUPP1 in IMCD3 cells.
ICC/IF / Mouse
Route of uptake of palmitoylated encephalitogenic peptides of myelin proteolipid protein by antigen-presenting cells: importance of the type of bond between lipid chain and peptide and relevance to autoimmunity.
ICC/IF / Mouse
Neurotrophins Redirect p75NTR from a clathrin-independent to a clathrin-dependent endocytic pathway coupled to axonal transport.
ICC/IF / Rat
Sorafenib functions to potently suppress RET tyrosine kinase activity by direct enzymatic inhibition and promoting RET lysosomal degradation independent of proteasomal targeting.
ICC/IF / Human
