ARG54252
anti-CD107b / LAMP2 antibody [H4B4] (APC)
anti-CD107b / LAMP2 antibody [H4B4] (APC) for Flow cytometry and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | APC-conjugated Mouse Monoclonal antibody [H4B4] recognizes CD107b / LAMP2 |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Ms, Rat |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody H4B4 recognizes CD107b / LAMP2, an extensively glycosylated 100120 kDa widely expressed lysosomeassociated protein. HLDA V.; WS Code P007 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | H4B4 |
| Isotype | IgG1 |
| Target Name | CD107b / LAMP2 |
| Antigen Species | Human |
| Immunogen | Human PBMC |
| Conjugation | APC |
| Alternate Names | CD antigen CD107b; LAMPB; CD107b; LGP110; Lysosome-associated membrane glycoprotein 2; LAMP-2; CD107 antigen-like family member B; Lysosome-associated membrane protein 2 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 0.02% Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P13473 Human Lysosome-associated membrane glycoprotein 2 |
|---|---|
| Gene Symbol | LAMP2 |
| Gene Full Name | lysosomal-associated membrane protein 2 |
| Background | CD107b (lysosome-associated membrane protein-2, LAMP-2), together with CD107a / LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, especially CD107b is important regulator in successful phagosomal maturation. CD107b deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danons disease). Immature CD107b is an approximately 45 kDa protein, but after extensive glycosylation the mature glycoprotein has about 100-120 kDa. |
| Function | Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 45 kDa |
| PTM | O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. |
Clone References
Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy.
WB, ICC/IF / Human
Amino acids activate mammalian target of rapamycin (mTOR) complex 1 without changing Rag GTPase guanyl nucleotide charging.
Efficient intracerebral delivery of AAV5 vector encoding human ARSA in non-human primate.
IHC-FoFr / Macaque Monkey
Autophagy and exosomes in the aged retinal pigment epithelium: possible relevance to drusen formation and age-related macular degeneration.
FACS / Human
Identification of the SPG15 gene, encoding spastizin, as a frequent cause of complicated autosomal-recessive spastic paraplegia, including Kjellin syndrome.
ICC/IF / African Green Monkey
