ARG62704

anti-CD108 antibody [MEM-150]

anti-CD108 antibody [MEM-150] for Flow cytometry,Immunoprecipitation,Western blot and Human

Cell Biology and Cellular Response antibody; Immune System antibody; Neuroscience antibody

Overview

Product Description

Mouse Monoclonal antibody [MEM-150] recognizes CD108

Tested Reactivity Hu
Tested Application FACS, IP, WB
Specificity The clone MEM-150 reacts with CD108 (JMH blood group antigen), a 80 kDa GPI-anchored glycoprotein expressed on various cell types including erythrocytes, lymphoblasts; at low levels it is present on circulating lymphocytes.
HLDA V; WS Code AS S017
HLDA V; WS Code BP BP347
HLDA VI; WS Code BP 401
HLDA VI; WS Code BP 475
HLDA VI; WS Code NL N-L156
HLDA VI; WS Code P PR-65
Host Mouse
Clonality Monoclonal
Clone MEM-150
Isotype IgM
Target Name CD108
Antigen Species Human
Immunogen HPB-ALL human T cell line
Conjugation Un-conjugated
Alternate Names H-Sema-L; Sema L; Semaphorin-7A; Sema K1; CDw108; SEMAK1; CD antigen CD108; CD108; John-Milton-Hargen human blood group Ag; Semaphorin-L; Semaphorin-K1; JMH; H-SEMA-K1; SEMAL; JMH blood group antigen

Application Instructions

Application Suggestion
Tested Application Dilution
FACS4 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by precipitation methods and ion exchange chromatography.
Purity > 95% (by SDS-PAGE)
Buffer TBS (pH 8.0) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8482 Human SEMA7A

Swiss-port # O75326 Human Semaphorin-7A

Gene Symbol SEMA7A
Gene Full Name semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
Background CD108 (Sema7A) is a GPI-anchored semaphorin family member, which enhances central and peripheral axonal growth and is required for proper axon track formation during ebryogenesis. CD108 also regulates osteoclast differentiation and pre-osteoblastic cell migration, and in immune system affects cell proliferation, chemotaxis and cytokine release. On erythrocytes CD108 defines the JMH (John-Milton-Hagen) human blood group. CD108 signalizes through its receptors – plexin C1 and beta1 integrins.
Function Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Immune System antibody; Neuroscience antibody
Calculated MW 75 kDa

Clone References

Semaphorin 7A contributes to West Nile virus pathogenesis through TGF-β1/Smad6 signaling.

WB, ICC/IF / Mouse

Sultana H et al.
J Immunol.,  (2012)

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Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.

Drukker M et al.
Nat Biotechnol.,  (2012)

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Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules.

Angelisová P et al.
Immunobiology.,  (1999)

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Evidence that CDw108 membrane protein bears the JMH blood group antigen.

Mudad R et al.
Transfusion.,  (1995)

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