ARG22709
anti-CD11a + CD18 antibody [CVS9]
anti-CD11a + CD18 antibody [CVS9] for IHC-Frozen sections,Flow cytometry and Horse
Overview
| Product Description | Mouse Monoclonal antibody [CVS9] recognizes CD11a + CD18 This antibody recognizes Equine homolog of the Human CD11a/CD18 cell surface antigen, a hetrodimer expressed on all equine cells of haemopoietic origin. Studies have indicated that equine CD11a/CD18 has higher expression on some subpopulations of T-cells. |
|---|---|
| Tested Reactivity | Hrs |
| Tested Application | FACS, IHC-Fr |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CVS9 |
| Isotype | IgG1 |
| Target Name | CD11a + CD18 |
| Antigen Species | Horse |
| Immunogen | Equine leucocytes. |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA1A; LFA-1A; Integrin alpha-L; CD11A; Leukocyte function-associated molecule 1 alpha chain; LFA-1; CD11 antigen-like family member A; CD antigen CD11a |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | ITGAL |
|---|---|
| Gene Full Name | integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) |
| Background | ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. [UniProt] |
| Calculated MW | 129 kDa |
| PTM | In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents. |
