ARG20832
anti-CD11a / LFA1 alpha antibody [I21/7] (PE)
anti-CD11a / LFA1 alpha antibody [I21/7] (PE) for Flow cytometry,IHC-Frozen sections,ICC/IF,Blocking and Mouse
Overview
Product Description | PE-conjugated Rat Monoclonal antibody [I21/7] recognizes CD11a / LFA1 alpha |
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Tested Reactivity | Ms |
Tested Application | BL, FACS, ICC/IF, IHC-Fr |
Specificity | Mouse CD11a. The clone I21/7 reacts with the α subunit of mouse CD11a, also known as LFA-1, and a member of the integrin family of cell adhesion molecules. The antigen is expressed on thymocytes, T and B lymphocytes, and on non-lymphoid cells in the bone marrow. |
Host | Rat |
Clonality | Monoclonal |
Clone | I21/7 |
Isotype | IgG2a, kappa |
Target Name | CD11a / LFA1 alpha |
Antigen Species | Mouse |
Immunogen | Whole BW5147 cells |
Conjugation | PE |
Alternate Names | Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA1A; LFA-1A; Integrin alpha-L; CD11A; Leukocyte function-associated molecule 1 alpha chain; LFA-1; CD11 antigen-like family member A; CD antigen CD11a |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Buffer | PBS, 0.1% Sodium azide and Sucrose. |
Preservative | 0.1% Sodium azide |
Stabilizer | Sucrose |
Concentration | 0.1 mg/ml |
Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | ITGAL |
Gene Full Name | integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) |
Background | ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Function | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. [UniProt] |
Calculated MW | 129 kDa |
PTM | In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents. |