ARG62710
anti-CD11a / LFA1 alpha antibody [M17 / 4] (FITC)
anti-CD11a / LFA1 alpha antibody [M17 / 4] (FITC) for Flow cytometry and Mouse
Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | FITC-conjugated Rat Monoclonal antibody [M17/4] recognizes CD11a / LFA1 alpha |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS |
| Specificity | The clone M17/4 reacts with CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | M17/4 |
| Isotype | IgG2a |
| Target Name | CD11a / LFA1 alpha |
| Antigen Species | Mouse |
| Immunogen | C57BL/6 mouse splenic secondary cytotoxic T lymphocytes |
| Conjugation | FITC |
| Alternate Names | Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA1A; LFA-1A; Integrin alpha-L; CD11A; Leukocyte function-associated molecule 1 alpha chain; LFA-1; CD11 antigen-like family member A; CD antigen CD11a |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 0.5 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. |
| Research Area | Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 129 kDa |
| PTM | In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents. |
