ARG62710

anti-CD11a / LFA1 alpha antibody [M17 / 4] (FITC)

anti-CD11a / LFA1 alpha antibody [M17 / 4] (FITC) for Flow cytometry and Mouse

Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description FITC-conjugated Rat Monoclonal antibody [M17/4] recognizes CD11a / LFA1 alpha
Tested Reactivity Ms
Tested Application FACS
Specificity The clone M17/4 reacts with CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
Host Rat
Clonality Monoclonal
Clone M17/4
Isotype IgG2a
Target Name CD11a / LFA1 alpha
Antigen Species Mouse
Immunogen C57BL/6 mouse splenic secondary cytotoxic T lymphocytes
Conjugation FITC
Alternate Names Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA1A; LFA-1A; Integrin alpha-L; CD11A; Leukocyte function-associated molecule 1 alpha chain; LFA-1; CD11 antigen-like family member A; CD antigen CD11a

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16408 Mouse ITGAL

Background CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions.
Research Area Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 129 kDa
PTM In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents.