ARG62711

anti-CD11a / LFA1 alpha antibody [M17 / 4] (Functional Grade)

anti-CD11a / LFA1 alpha antibody [M17 / 4] (Functional Grade) for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation and Mouse

Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Functional grade and low endotoxin Rat Monoclonal antibody [M17/4] recognizes CD11a / LFA1 alpha
Tested Reactivity Ms
Tested Application FACS, FuncSt, IHC-Fr, IP
Specificity The clone M17/4 reacts with CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
Host Rat
Clonality Monoclonal
Clone M17/4
Isotype IgG2a
Target Name CD11a / LFA1 alpha
Antigen Species Mouse
Immunogen C57BL/6 mouse splenic secondary cytotoxic T lymphocytes
Conjugation Un-conjugated
Alternate Names Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA1A; LFA-1A; Integrin alpha-L; CD11A; Leukocyte function-associated molecule 1 alpha chain; LFA-1; CD11 antigen-like family member A; CD antigen CD11a

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/ml
FuncStAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
Application Note IHC-Fr: Acetone fixation.
Functional studies: Blocking.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-Fr: Mouse spleen or thymus

Properties

Form Liquid
Purification Purification with Protein G.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16408 Mouse ITGAL

Background CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions.
Research Area Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 129 kDa
PTM In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents.