ARG62705

anti-CD11a / LFA1 alpha antibody [MEM-83] (low endotoxin)

anti-CD11a / LFA1 alpha antibody [MEM-83] (low endotoxin) for Functional study,Flow cytometry,Immunoprecipitation and Human

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [MEM-83] recognizes CD11a / LFA1 alpha
Tested Reactivity Hu
Tested Application FACS, FuncSt, IP
Specificity The antibody MEM-83 reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
Host Mouse
Clonality Monoclonal
Clone MEM-83
Isotype IgG1
Target Name CD11a / LFA1 alpha
Antigen Species Human
Immunogen Human peripheral blood lymphocytes.
Conjugation Un-conjugated
Alternate Names Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA1A; LFA-1A; Integrin alpha-L; CD11A; Leukocyte function-associated molecule 1 alpha chain; LFA-1; CD11 antigen-like family member A; CD antigen CD11a

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/ml
FuncStAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein.
Buffer PBS
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3683 Human ITGAL

Swiss-port # P20701 Human Integrin alpha-L

Gene Symbol ITGAL
Gene Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Background ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is a receptor for F11R (PubMed:11812992, PubMed:15528364). Integin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed:29100055). Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992). Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis (By similarity). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590). [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 129 kDa
PTM In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents. [UniProt]

Clone References

LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro.

BL / 

Ghislin S et al.
BMC Cancer.,  (2012)

publication_link

 

hr_line

Regulation of LFA-1-dependent inflammatory cell recruitment by Cbl-b and 14-3-3 proteins.

Choi EY et al.
Blood.,  (2008)

publication_link

 

hr_line

A novel LFA-1 activation epitope maps to the I domain.

Landis RC et al.
J Cell Biol.,  (1993)

publication_link

 

hr_line

Involvement of the I domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3.

Landis RC et al.
J Cell Biol.,  (1994)

publication_link

 

hr_line